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Direct chemiluminescence commonly used luminescent agent is
Acridine ester and ruthenium tripyridine.

Acridine ester is a chemical substance that can be used as a chemiluminescence marker. The emission intensity reaches the maximum at about 0.4s and the half-life is about 0.9s after adding luminescent starting reagent. ..

The luminescence principle of ruthenium bipyridine as a label is a specific chemiluminescence reaction initiated by electrochemistry on the electrode surface.