Luminol reagent was originally used to detect protein. In western blot, enzyme-linked antibodies are commonly used to bind to the protein to be detected, and luminol chemiluminescent reagents are combined with enzymes (peroxidase) to make local luminescence and show the position of the protein. The luminescence in the picture is very bright, which should be due to the addition of chemiluminescence enhancer in the reagent. Of course, the actual detection of protein, the light is far from so bright.

In addition, the derivatives of luminol, such as Isoluminol (ABEI), were labeled on carboxylic acids and ammonia compounds, separated by high performance liquid chromatography (HPLC) or liquid chromatography (LC), and then reacted with hydrogen peroxide-potassium ferricyanide under alkaline conditions for chemiluminescence detection. For example, the newly synthesized chemiluminescence reagent isoluminol isothiocyanate was labeled on yeast RNA, separated by centrifugation and dialysis, and then detected by chemiluminescence.

Desheng's current chemiluminescence reagents include acridine ester and luminol. And the quality is guaranteed and the supply is sufficient.