(1) polymerase chain reaction;
(2)DNA sequencing;
(3) fluorescence in situ hybridization;
(4)DNA imprinting;
(5) Single nucleotide polymorphism;
(6) ligase chain reaction (LCR);
(7) Gene chip technology.
Among them, PCR products occupy the main market of molecular diagnosis at present, and gene chip is the main trend of molecular diagnosis market development. PCR products have high sensitivity, strong specificity and short diagnostic window, which can be used for qualitative and quantitative detection. Can be widely used in hepatitis, sexually transmitted diseases, pulmonary infectious diseases, eugenics, genetic diseases, tumors and so on. It fills the gap in the window period of early immune testing and provides effective help for early diagnosis, early treatment and safe blood use.
There are also reagents needed for molecular diagnosis, and Roche Bio-industry provides a variety of nucleic acid amplification enzymes. Taq
DNA polymerase is the most widely used and active PCR reaction polymerase at present. And AMV reverse transcriptase and M-MLV reverse transcriptase produced according to GMP specifications provided by Roche. Reverse transcriptase is the basis for the success of RT-PCR reaction and other amplification techniques.