On January 19, 2022, Yan Huabing’s team from the Institute of Economic Crops of the Guangxi Academy of Agricultural Sciences and Fraser Gene published a paper titled “Chromosomal-level genome” in the authoritative journal Horticulture Research (IF=6.79) in the field of horticulture. and multi-omics dataset of Pueraria lobata var. thomsonii provide new insights into legume family and the isoflavone and puerarin biosynthesis pathways" research paper, which constructed a high-quality chromosome-level genome of Pueraria lobata through PacBio and Hi-C sequencing. Analyzed the genomic characteristics of kudzu, and then used multi-omics technologies including genome, transcriptome, and metabolome to deeply analyze the biosynthetic mechanism of important secondary metabolites of kudzu, thus providing information for resource utilization and genetic breeding of kudzu. and other studies provide new insights.
In view of the high heterozygosity of kudzu, the researchers chose PacBio and Hi-C sequencing. The constructed kudzu genome size was 1.38Gb, Contig N50=598 kb, and 99.3% of the sequences were anchored. On 11 chromosomes, BUSCO assessed the genome integrity as 92.9%. Through annotation, *** obtained 45,270 protein-coding genes, 94.4% of which can be functionally annotated, and repetitive sequences account for 62.7% of the genome.
Comparative genomic analysis of kudzu and 16 closely related species (including 5 leguminous plants) showed that:
By comparing high puerarin ZG-19 and low puerarin Transcriptome and metabolome analysis of ZG-39 was performed. The researchers detected 614 225 differential metabolites (DMs) and 1814 differentially expressed genes (DEGs). The rich functional categories of DMs and DEGs overlapped, indicating that they are all Genes or metabolites related to flavonoids, isoflavones and ABC transport.
Further analysis of the correlation coefficient between metabolites and gene expression showed that metabolites and gene pairs were highly correlated in the samples, with 60% of the significant correlations involving up-regulated metabolites and down-regulated or unchanged genes. In 15% of significant correlations, metabolite and gene expression changed in the same direction.
In addition, researchers have discovered a large number of DMs and DEGs in the isoflavone biosynthetic pathway. This fully explains the biosynthetic pathway of isoflavones in kudzu.
Through homologous gene search, the researchers found that 9 gene families encoding key enzymes in the puerarin synthesis pathway have all been expanded in kudzu; by analyzing the glycosyltransferase family that catalyzes glycosylation Modified genes, 104 GT genes were finally identified, 13 genes were homologous to 8-C-glucosyltransferase (8-C-GT), 6 of which were previously studied to catalyze daidzein C- Homologous to the PIUGT43 gene that is glycosylated to puerarin.
The gene (CHR11G3854.1) encoding soybean isoflavone synthase (IFS) catalyzes the synthesis of daidzein, an intermediate metabolite in the synthesis of puerarin, and has been identified as highly related to the synthesis pathway of puerarin. In summary, the above analysis preliminarily analyzed the biosynthetic pathway of puerarin in kudzu.
In summary, this study analyzed the evolutionary characteristics of the kudzu genome by constructing a high-quality kudzu genome; and deeply analyzed the important secondary metabolites isoflavones and puerarin in kudzu through multi-omics analysis. and other biosynthetic pathways, thus providing new insights into research on resource utilization and genetic breeding of kudzu.
In recent years, the team of researcher Yan Huabing from the Institute of Economic Crops of the Guangxi Academy of Agricultural Sciences has continued to carry out joint research with Huazhong Agricultural University, Fraser Gene, Shanghai University, Guangxi University of Traditional Chinese Medicine, Guangxi Medical University and other units to develop Pueraria lobata globally. A series of achievements have been achieved in resource collection and identification evaluation, Pueraria genus resource classification, Pueraria genome and molecular biology, pink kudzu and wild kudzu variety breeding, healthy seedling production, and high-yield and efficient cultivation.
So far, the team has extensively collected 419 global kudzu germplasm resources, including kudzu, pink kudzu, kudzu, kudzu, big flower kudzu, tiger kudzu, bitter kudzu, red kudzu, Sumeru kudzu, edible kudzu, etc.; passed Developed kudzu SSR molecular markers and constructed the Guangxi kudzu core germplasm library; analyzed the metabolism in the roots of three varieties of kudzu species including kudzu, kudzu, and kudzu that affect the edible quality and medicinal quality through extensive targeted metabolomics Differences; through chloroplast genome research combined with phenotypic identification, the phylogenetic relationship between Pueraria lobata and its closely related species was revealed; structural genes and transcription factors related to the regulation of puerarin synthesis and metabolism were discovered, and relevant gene function verification work is being carried out; A series of new kudzu varieties suitable for the development of kudzu tea, high-puerarin kudzu, residue-free kudzu, and medicinal kudzu have been selected and developed, and supporting seedling breeding and efficient cultivation technologies have been gradually established. The above research-related results have been published in Horticulture Research, Frontier in Plant Science, Molecules, Journal of Plant Genetic Resources, Acta Plant Physiology and other journals. Related research has been funded by projects from the National Natural Science Foundation of China, Guangxi Department of Science and Technology and other departments. The publication of the kudzu genome article will further promote the evolution and classification research of kudzu plants around the world, promote the scientific and technological progress of the kudzu industry in my country, and play the role of basic research source supply to further promote the high-quality development of the kudzu industry with local characteristics and advantages in Guangxi.
Speaking of kudzu, everyone must be familiar with it. It was first used for ecological management in the United States and later became widespread and was classified as an invasive organism. The Thai kudzu industry and its health benefits are popular all over the world. The earliest documented records about kudzu appeared in the Zhou Dynasty. "Shen Nong's Materia Medica" records that "(Kudzu root) is responsible for quenching thirst, severe body heat, vomiting, and various paralysis, arouses yin energy, and relieves various poisons." Pueraria lobata has the functions of relieving muscles and reducing fever, producing body fluids and quenching thirst, clearing up rash, promoting yang and stopping diarrhea, dredging menstruation and activating collaterals, and detoxifying alcohol. Modern pharmacological research shows that Pueraria lobata has good pharmacological activities in improving the cardiovascular system, antioxidant, hypoglycemic, antipyretic, anti-inflammatory, anti-alcoholic and liver protection, neuroprotective, anti-osteoporosis and estrogen-like effects.
Pueraria lobata is a plant of the Fabaceae family. It is a medicinal and food plant. It is known as "Asian ginseng" and "Southern ginseng and northern ginseng". It is widely planted in Guangxi, Guangdong, Jiangxi, Hunan, Hubei and other places, among which Guangxi is the main planting area of ??kudzu, with the planting area ranking first in the country! Among them, Heping Town, Teng County, Wuzhou is the famous "Hometown of Kudzu" in China, and Teng County Pueraria Tianxiang and Heping Pueraria Industry (Core) Demonstration Zone have been rated as Guangxi Modern Characteristic Agriculture (Core) Four-Star Demonstration Zone. At present, the development of Guangxi's kudzu industry still faces many problems that need to be solved. The analysis of the kudzu genome will provide scientific and technological support for the high-quality development of the kudzu industry.
Reprinted from: /s/wJkSj2pVJAQibio3unCMEw