In 1994, in order to save the wild Chinese soft-shell turtle germplasm resources of the Yellow River strain, Dingma Farm sent a large number of technicians to start collecting Chinese soft-shell turtles of the Yellow River strain. In the absence of national and industry standards, a draft was formulated Strict selection standards for original species of wild Chinese soft-shell turtles, 42,000 original species of wild Chinese soft-shelled turtles were carefully collected from the four river systems of the Yellow River, Ancient Canal, Majia River, and Tuhai River, and were placed in natural ecological ponds according to the river systems. Breeding and cultivation in breeding ponds, and optimization again. The Shandong Provincial Aquatic Products Original and Improved Variety Approval Committee concluded that the selected wild Chinese soft-shelled turtle is the original species. This has made a huge contribution to China's preservation and protection of wild Chinese soft-shell turtle germplasm resources in the Yellow River Basin, and also laid the foundation for high-quality Chinese soft-shell turtle breeding in China.
After selecting wild Chinese soft-shelled turtles, Dingma Technology Company conducted research and development on high-tech and high-quality Chinese soft-shelled turtle breeding technology. The preferred wild Chinese soft-shelled turtle species from the four river systems are first used to self-propagate, and the wild offspring parent Chinese soft-shelled turtles are preferably cultivated. The wild offspring parent Chinese soft-shelled turtles are used to mate with each other to breed non-related and genetically identical "Chinese soft-shelled turtles". "Huangyun" brand Chinese soft-shelled turtle varieties and new parent species, thus creating the "425-1-30" high-quality Chinese soft-shelled turtle breeding technology. The Chinese soft-shelled turtle breeding technology has a cycle of 30 years, and each cycle has 4 breeding periods and 2 breeding periods. Among them, the breeding period of the parent Chinese soft-shelled turtle is 5 years and the breeding period is 10 years. In each breeding period, it is concluded that There are four types of Chinese soft-shelled turtles that are not directly related to each other and the parent improved Chinese soft-shelled turtles. The advanced Chinese soft-shelled turtles breeding technology not only optimizes the Yellow River strain Chinese soft-shelled turtles, but also avoids the deformities, breed degradation, and poor quality of Chinese soft-shelled soft-shelled turtles caused by inbreeding of Chinese soft-shelled turtles. Shortcomings such as low immunity, low survival rate, and slow growth ensure the quality, stability, and safety of the Chinese soft-shelled turtle, thereby providing technical support for the protection and breeding of high-quality Chinese soft-shelled turtle parent species and improved varieties of the Yellow River strain.
In order to protect the excellence, stability and safety of the Yellow River strain of Chinese soft-shelled turtle, they established a scientific germplasm identification method to ensure that the "425-1-30" Chinese soft-shelled turtle is cultivated with advanced breeding technology. "Huangyun" brand Chinese soft-shelled turtle is an improved species. The method of combining morphological measurement and structural observation was used for seed selection; the isoenzyme method was used for biochemical analysis to determine the biochemical genetic characteristics and genetic structure of the cultivated "Huangyun" brand Chinese soft-shelled turtle varieties to maintain the safety and quality of the original germplasm. Stability; using polymerase chain reaction (PCR) technology and restriction length polymorphism (RELP) method, the cytochrome (cyt) gene of mitochondrial DNA (mtDNA) of multiple Chinese soft-shell turtle populations was analyzed, and it was concluded that "Huang Yun" "Huangyun" brand Chinese soft-shelled turtle has obvious divergence in its genome from other groups of soft-shelled turtles. The population genetic diversity is high and its growth rate is fast. Two-dimensional electrophoresis technology was used to analyze the specific protein of Chinese soft-shelled turtle muscle tissue, and it was concluded that the "Huangyun" brand Chinese soft-shelled turtle was The protein is significantly higher than that of other Chinese soft-shelled turtles, indicating that its genetic diversity is retained.
The "Huangyun" brand Chinese soft-shelled turtle, bred using the "425-1-30" high-quality Chinese soft-shelled turtle seed breeding technology, was compared with wild Chinese soft-shelled turtle original seed generation from 2000 to 2003. "Huangyun" brand Chinese soft-shell turtle has the characteristics of large body lamellae, large and thick skirt, khaki or dark brown carapace, light yellow ventral carapace, strong constitution, flexible movement, strong immunity, fast growth rate and high survival rate.
In 2005, the wild Chinese soft-shelled turtle original species and the "Huangyun" high-quality Chinese soft-shelled turtle seedlings selected by Dingma Technology Company were approved by the Shandong Provincial Aquatic Products Original and Improved Variety Approval Committee as the Chinese soft-shelled turtle original species and the "Huangyun" high-quality Chinese soft-shelled turtle seedlings, respectively. The improved varieties were certified as Shandong Provincial Chinese Soft Turtle Original Breeding Farm in 2006 and as national-level Chinese Soft Turtle Original Breeding Farm in 2007.
In March 2005, the "425-1-30" Chinese soft-shelled turtle breeding technology formulated by Dingma Technology Company was evaluated by the Shandong Provincial Department of Science and Technology as a "domestic first"; " Research on high-quality Chinese soft-shell turtle breeding technology and industrialized breeding" won the second prize of Shandong Province Science and Technology Progress Award; in 2005, the company's "High-quality Chinese soft-shell turtle breeding and green breeding high-tech industrialization project" was listed in the National High-tech Industrialization Demonstration project; the "Yellow River Strain Chinese Soft Turtle Breeding Demonstration Project" was listed as a modern agriculture demonstration project by the National Development and Reform Commission; in 2006, the "Soft-shell Turtle Seed Breeding and Aquaculture High-Tech Development" was listed as a national Spark Plan project by the Ministry of Science and Technology.
Dingma Biotechnology Co., Ltd. has scientifically and boldly explored and developed Chinese soft-shell turtle breeding, and summarized a set of new technologies and standardized operating procedures for Chinese soft-shell turtle breeding.
The wild parent Chinese soft-shelled turtle, the first and second generation "Huangyun" brand wild parent Chinese soft-shelled turtle, and the finished Chinese soft-shelled turtle are cultivated and bred using natural ecological biological green technology. The breeding water uses deep groundwater, and the water quality complies with the national standards. The water quality standards of breeding water should be properly matched with the stocking of silver carp to adjust the content of phytoplankton and plants, keep the water quality relatively stable and fresh, create a good growth environment for soft-shell turtles, and create a modern new technology for raising soft-shell turtles with water. . The feed is fed at the right time and in the right amount with high-quality, full-price feed that meets the national green food standards, providing reliable technical support for the green and healthy breeding of Chinese soft-shell turtles in China. Comprehensive epidemic prevention technology is used to prevent Chinese soft-shell turtle disease. National banned drugs are not used in Chinese soft-shell turtle breeding, and limited drugs are used in small quantities or to avoid. The specific epidemic prevention technical measures are: clearing ponds and disinfecting; doing ecological water diversion; maintaining breeding water level; regular water disinfection; strict control of feeding materials; regular disinfection of tools, personnel, and the environment; regular prevention of drugs in the body, etc. Chinese soft-shell turtle disease prevention is carried out in strict accordance with the regulations of the Ministry of Agriculture NY/T5071 for timely targeted prevention. The prescribed amount of antibiotics is added to the feed and fed once a month for 5 days each time, or Chinese herbal powder is added to the feed for feeding. Twice a month, each time for 5-7 days, or often add microecological preparations to the feed to inhibit the reproduction of pathogenic microorganisms in the body, maintain the ecological balance in the body, regulate intestinal digestive function, and improve the quality of the feed. Transform absorption rate, enhance immunity, enable healthy growth, and ensure the quality of Chinese soft-shelled turtle green food. In 2001, the company's Chinese herbal medicine for the prevention and treatment of turtle disease was awarded the "Excellent Project Achievement Award" by the state.
Since 2001, the company’s “Huangyun” brand Chinese soft-shelled turtle cultured using natural ecological biological green technology has been certified by the China Green Food Development Center as China’s first aquatic series “A” grade green food; 2002 In 2005, it was rated as the "Best-Selling Product Award" at the China Green Food Fuzhou Expo; in 2005, it was certified as one of the top ten green food brands in Shandong Province by the Shandong Green Food Development Center; in 2007, it was certified as a famous brand agricultural product in Shandong Province; "Huangyun" brand trademark has been certified as a famous trademark in Shandong Province and is deeply trusted and favored by Chinese consumers.
Years of production practice and technical research have enabled Dingma Technology Company to successfully summarize natural temperature control, humidity control, and ecological hatching technologies in the breeding of improved Chinese soft-shell turtle species and high-quality Chinese soft-shell turtle breeding. Temperature-controlled ecological breeding technology and juvenile turtle insulation and constant temperature intensive cultivation technology have resulted in a hatching rate of over 98%. The juvenile turtles cultivated through intensive insulation and constant temperature cultivation are strong and gain weight faster than ordinary temperature-controlled breeding. The weight of the young turtles increases by 1.5-1.7 times, and then they are transferred to natural ponds for easy rearing, with fast growth, good quality, high yield and great benefits. These breeding technologies have been appraised by the Shandong Provincial Department of Science and Technology and are among the leading in the country.
Dingma Biotechnology Co., Ltd. uses advanced breeding and breeding technology to grow the company and become the only original breeding farm of Chinese soft-shell turtle of the Yellow River strain in China. In 2007, 4.5 million "Huangyun" brand high-quality Chinese soft-shelled turtle seedlings were bred, with an annual output of 400,000 kilograms of "Huangyun" brand high-quality Chinese soft-shelled turtles, driving more farmers to become rich and greatly promoting the development of high-quality Chinese soft-shelled turtles in China. The development of the breeding industry has created higher economic benefits.
The whole body of the turtle is a treasure, and the turtle shell, head, meat, blood, gallbladder, etc. can be used as medicine. According to the "Compendium of Materia Medica", turtle shell glue can soften hardness and disperse stagnation, activate blood circulation and remove blood stasis, nourish yin, and nourish blood. , reduce fever and eliminate blood stasis; turtle meat can treat chronic dysentery, tuberculosis, athlete's foot and other diseases; turtle shells are mainly used to treat bone steaming fever, yin deficiency and wind movement, hepatosplenomegaly and liver cirrhosis; external application of turtle blood can treat facial nerve paralysis, Children with malnutrition have hot flashes and have the function of rapid contraction and bleeding. Mixing wine with it can cure hemorrhagic tuberculosis in women; turtle eggs can cure chronic diarrhea and dysentery; turtle bile has the effect of curing hemorrhoids and fistulas; the dried turtle head is used as medicine and is called "Turtle Head" , can cure anal prolapse, leakage, etc. There are as many as 8 types of medicinal parts recorded in the "Dictionary of Traditional Chinese Medicine". Turtle is also considered to be a very effective aphrodisiac. Research from the Faculty of Pharmacy of the University of Tokyo in Japan believes that eating turtle can quickly eliminate fatigue. In recent years, athletes have been encouraged to take turtle soup before competitions.
While developing fine breed breeding and green farming scale, in 2003, the company cooperated with the General Hospital of the People's Liberation Army of China and began to use high-quality tenma soft-shell turtle to conduct technical research and development of soft-shell turtle health food, using domestically advanced multi-stage counter-current extraction technology to extract soft-shell turtle Glue, use nano-homogeneous spray drying technology to obtain turtle glue powder, use fluidized bed airflow powder milling technology to obtain turtle bone powder, use vacuum drying and supercritical extraction technology to obtain turtle oil, and use advanced food turtle technology to process turtle convenience foods. Functional studies have shown that the turtle gum series health food has the functions of anti-fatigue, improving immunity, anti-radiation, and enhancing physical fitness. Further research has shown that the turtle gum series health food also has the functions of softening and dispersing hard knots, preventing cancer, lowering blood sugar, softening blood vessels, and beautifying the skin. and other medicinal and health-care values. This series of health food is deeply processed using original domestic technology, which greatly improves the content, biological activity and efficacy of the active ingredients of the product, filling the gaps at home and abroad. The "Research and Development of Turtle Maw" project passed the Shandong Provincial Scientific and Technological Achievements Appraisal and won the third prize of the Shandong Provincial Science and Technology Progress Award.