The area containing the reaction plate is a detection component, which contains a blood coagulation channel and a chromogenic substrate channel or an immunoturbidimetric channel;

Scattering turbidimetry: all ACL systems have it. Condensation formation was detected by light scattering at 660nm wavelength.

Chromogenic substrate method/immunoturbidimetric method: 405nm, and the absorbance was detected.

2. Detection principle

1) was detected by turbidity method (coagulation method)

Scattering turbidimetry (coagulation method) detection refers to detecting and recording the coagulation time of plasma samples. This technology determines the end point of curing by detecting the change of light scattering.

Scattering turbidimetry/coagulation method, when the sample reacts with the reagent, fibrinogen is converted into fibrin, and light passes through the reaction medium, thus light scattering occurs. 660nm light passes through the plasma, and 900 angle is equipped with an optical sensor to detect the optical signal.

The coagulation process of fibrinogen is accompanied by the enhancement of light scattering signal. With the change of the detected optical signal, the electrical signal detected by the photodetector also changes. The changing electrical signal determines the solidification end point through a series of mathematical algorithms of the software.

2) chromogenic substrate method (Figure: indirect chromogenic substrate method)

Chromogenic substrate method can be divided into direct chromogenic substrate method and indirect chromogenic substrate method.

Direct method: the analyte directly binds to a specific chromogenic substrate. For example: protein C, plasminogen PLG.

Indirect method: by improving the detection system, adding excessive reactive enzymes and substances that can bind to analytes, and finally using specific synthetic substrates to detect the activity of the remaining enzymes, so as to achieve the purpose of detection. For example: heparin, AT-III.

In most cases, the absorbance of p-nitroaniline (pNA) in the synthetic substrate was detected at 405nm.

The chromogenic substrate channel uses the colorimetric principle to detect the absorbance change value in the reaction cup. The 405nm light source passes through the reaction cup and is received by the optical sensor. The absorbance in the reaction cup is directly proportional to the concentration of pNA. The optical signal received by the optical path detector is converted into an electrical signal, which is directly proportional to the activity of the enzyme.

3) Immunoturbidimetry

Immunoturbidimetry refers to the direct detection and recording of analyte concentration. This method detects the physical concentration of analyte by detecting the change of optical density value, but not its activity. Like transmittance turbidimetry, immunoturbidimetry relies on the formation of antigen-antibody complex to detect the change of transmittance.

ACLTM 8/9/ 10000 system detects the optical density of the 405nm channel and compares it with the reference emulsion.

Using the wavelength of 405nm, the absorbance value (Δ a) in the reaction cup was detected. The light transmitted through the reaction cup is detected by a light sensor. The amount of light signal absorbed by the liquid in the reaction cup is directly proportional to the concentration of antigen-antibody complex. The optical signal received by the optical path detector is converted into an electrical signal, which is directly proportional to the activity of the enzyme.