At present, frequent immunization and abuse of antibiotics in the intensive breeding industry of pigs and chickens have led to severe drug resistance of pathogenic bacteria. Focusing on this major theoretical issue, great progress has been made in three aspects:

(1) Distinguish between drug resistance and tolerance Drug properties, and put forward the theory of drug resistance dynamics of pathogenic bacteria groups. Bacteria are independent cells with perfect evolution. Antibiotics are designed to inhibit their growth based on their key biochemical processes. Bacteria use their existing biochemical mechanisms to develop resistance to antibiotics. That is, bactericidal mechanisms and drug resistance mechanisms already exist. Antibiotic induction and Screened for drug resistance. According to the drug resistance regulatory mechanism and whether genetic mutations occur, drug resistance phenotypes are divided into drug resistance mutations and drug resistance adaptation. Linear gradient plates are used to distinguish bacterial resistance and drug resistance changes under the action of antibiotics, and through genomics, Proteomics, drug accumulation, and ultrastructure have provided a detailed description of drug resistance, and explored drug resistance and drug resistance regulatory mechanisms. A research model on the dynamics of pathogenic bacterial population resistance was established after post-oral inoculation of chicken cecal flora and the application of pesticides to study the effects of different antibiotics on the dynamic process of the generation and elimination of intestinal flora resistance.

(2) Improve stable, accurate and fast drug susceptibility test methods and establish a drug resistance monitoring network. For the first time, the concentration-sterilization curve equation describing antibiotic bactericidal kinetics and the isoboloid method describing the simultaneous action of multiple factors on bactericidal power were established; antibiotic susceptibility test kits (ZL200620080330.7) and 96-dot array drugs were developed successively. Sensitivity test kit (ZL200820187541. Large-scale breeding and processing groups such as Jinluo, Yinbao, and Delis have established a drug resistance monitoring network for animal-derived pathogenic bacteria in Shandong Province to guide the rational use of veterinary drugs in Shandong Province to reduce drug resistance and drug residues.

(3) Early warning and prevention technology of high resistance and multiple resistance mechanisms. Drug resistance is an ecological problem. Among the potential intestinal and environmental flora, highly resistant and multi-resistant strains are the most harmful. We have established a prediction and early warning research system for MRSA, VRE, NDM-1, and cfr, and researched multi-resistant inhibitors; we are the first to develop them in China. The successful liquid phytase and compound enzymes and their pellet feed post-spraying equipment have been rapidly promoted and used by more than a thousand units in Liuhe Group, New Hope Group and large pellet feed factories in various provinces, advancing the application level of feed enzymes in my country. Promote antibiotic substitution measures such as traditional Chinese medicine, compost, fermentation bed pig houses, fermented feed, microbial preparations, and enzyme preparations.

Undertake more than ten scientific research projects including the National Natural Science Foundation, national science and technology support, public welfare industry science and technology projects, 863 plan projects, Shandong Provincial Natural Science Foundation, Doctoral Fund, and Shandong Provincial Major Projects for the Transformation of Independent Innovation Achievements. , published more than 40 articles, including 11 SCI citations, published in Antimicrobial Agents and Chemotherapy, Foodborne Pathogens and Disease, Annals Microbiology and "Science China". Published 5 professional books. Translated the professional books on antibiotic resistance, Bacterial Resistance to Antimicrobials and Bacterial Virulence.