ApplicationNo.: 2009 10 130875.2 Application Date: April-65438+July 2009.
The invention belongs to the field of molecular biology DNA marker technology and application, in particular to a screening method of Porphyra yezoensis microsatellite markers and a method for analyzing genetic diversity of germplasm resources by using these markers, which lays a foundation for genetic structure analysis, germplasm resource protection and molecular marker-assisted breeding of Porphyra yezoensis.
Applicant: China Ocean University.
Address: 266 100 No.238 Songling Road, Laoshan District, Qingdao, Shandong Province
Inventors (designers): Kong Fanna, Yunxiang Mao, Yang Hui, Wang Li.
Main classification number: c12p19/34 (2006.38+0) i.
Classification number: c12p19/34 (2006.01) IC12q1/68 (2006.01) IC/kloc-0.
2. A molecular marker-assisted selection breeding method for Porphyra haitanensis.
ApplicationNo.: 2009 10 130876.7 Application Date: April-65438+July, 2009.
The invention belongs to the technical field of Porphyra haitanensis molecular breeding, and particularly provides a method for establishing a Porphyra haitanensis molecular marker-assisted selection hybrid breeding system, which can quickly and effectively select strains with excellent characters, shorten the breeding process and provide a new breeding system for the development of Porphyra haitanensis breeding.
Applicant: China Ocean University.
Address: 266 100 No.238 Songling Road, Laoshan District, Qingdao, Shandong Province
Inventor (Designer): Yunxiang Mao Kong Fanna Wang Liyanghui.
Main classification number: a 01h1/04 (2006438+0) i.
Classification number; a 0 1h 1/04(2006.0 1)I a 0 1h 1/02(2006.0 1)I a 0 1g 33/02(2006.0 1)I c 12q 1/68(2006.066
3. Edwardsiella tarda live attenuated vaccine
ApplicationNo.: 2007 100 15285.6 Application Date: July 2007-1 1.
The invention relates to an attenuated vaccine for aquaculture bacterial diseases, in particular to an attenuated live vaccine of Edwardsiella tarda, which is a technology for preventing and treating aquaculture animal diseases. The ESRB gene of Edwardsiella tarda strain LSE40 is an attenuated mutant, and the mutant is mzlE40esrb, which is preserved in CGMCC, General Microbiology Center of China Microbial Species Preservation Management Committee, with the preservation number of CGMCCNO.2087 The mutant mzlE40esrb does not contain foreign antibiotic screening markers and foreign gene fragments. Compared with wild Edwardsiella tarda, the attenuated vaccine has obvious low toxicity and immune protection rate, and does not contain any exogenous antibiotic resistance markers and exogenous gene fragments. Experiments show that the live attenuated vaccine can effectively protect susceptible fish from pathogenic Edwardsiella tarda infection.
Applicant: Institute of Oceanography, China Academy of Sciences.
Address: 26607 17 Nanhai Road, Qingdao, Shandong Province
Inventor (Designer): Mo,,, Li Jie,
Main classification number: A61K39/02 (2006438+0) i.
Classification number: A 61K 39/02 (2006.01) Ia61K 47/04 (2006.01) Ia61P 3104.
Publication number: 10 1342367
Public Day: 2009-0 1- 14
Patent agency: Shenyang Keyuan Patent and Trademark Agency Co., Ltd.
Authorized Agents: Xu Zongfu, Zhou Xiumei.
4. Promoter of Arthrospira phycocyanin gene and its preparation method
Application number: 200310105416.1application date: 2003-10/6.
The invention discloses a promoter of phycocyanin β and α subunit genes of Arthrospira. The length of the DNA fragment is 427bp, and its sequence is described as the promoter of phycocyanin β and α subunit genes. The sequence contains the following functional factors: the main functional regions of-10 and -35, and the functional factors such as DOFCOREZM, EBOXBNNAPA, GT 1CONSENSUS, GTGANTG 10, IBOXCORE, etc. The promoter has a complete -35 region and-10 region, and the interval length between the two functional regions indicates that it is an efficient promoter, and an efficient and strong promoter can be used to realize the task of transcription stage of algae genetic engineering, improve the efficiency of foreign gene expression, and then obtain more gene products needed for improving and utilizing Arthrospira. The application of the promoter in the promoter detection vector-green fluorescent protein has achieved high expression and proved its exploitability.
Applicant: China Ocean University.
Address: No.5 Yushan Road, Shinan District, Qingdao, Shandong Province, 266003
Inventor (Designer): Zhang Xuecheng, Liu Jinjie, Sui Ren.
Main classification number: c12n1511.
Classification number: c12n1512n15/29c12p19/34c07h2104.
Legal status:
2006- 1 1-08: Authorization
Effectiveness of substantive examination
open
Sovereign term: 1, the promoter of phycocyanin β and α subunit genes of Arthrospira, is a DNA fragment in the operon sequence of phycocyanin gene, with the length of 1545bp, which is characterized in that the length of the DNA fragment is 427bp, and its sequence is described as the promoter of phycocyanin β and α subunit genes. The sequence characteristics of the promoter are as follows: tcattattaatgcttgta ttgaccacg gattcgctat taattcctctt tcaaaaaaaaaaaaaaaata60 tacttaggttttttttttttttttTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA. aaaaaaaaaaaaaaaaagggtt 120 gctgtgtat aaggatt cgaagagggtg agaagaagg cgaatgttgtga tttatgaagt 180 tgattaca tttataca tttatcaaa aaat tcttctcata aaccctgtag
Publication number: 1528892
Public Day: September 2004-15
Patent agency: Qingdao Haihao Intellectual Property Office Co., Ltd.
Authorized Agent: Han Zhendong