From 1986 to 1990, I participated in the special topic of low-phenol cotton breeding hosted by 1988.
From 199 1 to 1995, the eighth five-year plan low phenol cotton breeding project, the project host.
1996-2000 presided over the special topic of insect-resistant cotton breeding in the ninth five-year plan.
1995- 1997 special fund for the development of cotton production by the Ministry of Agriculture-screening new cotton varieties with low phenol and regulators.
From 1996 to 1998, the project of China Agricultural Science and Education Fund-bivalent insect-resistant gene introduction, host.
1998- 1999, Postdoctoral Science Foundation of China-Study on the Formation Mechanism of Gossypol in Plants, Moderator.
1998-2000, project of Zhejiang science and technology commission-research on improvement of new early-maturing cotton varieties, moderator.
1999-200 1, Zhejiang Natural Science Foundation-Molecular Marker Study on Pigment Gland Formation in Cotyledons of Cotton, Moderator.
1999-2002 assisted in hosting the key project of National Natural Science Foundation-molecular marker and gene mapping research on cotton quality, yield and resistance.
In 2000-2002, the National Natural Science Gene-Molecular Marker and Cotton Fusarium Wilt Resistance Assisted Breeding, Regulator
From 200/KLOC-0 to 2003, the Ministry of Agriculture launched a special fund for cotton production-creation of specific cotton germplasm resources.
In 20001-2005, the annual special cotton breeding technology and new variety breeding participated in the sub-project of 863 project.
From 2002 to 2005, in the 863 project year, he presided over the breeding of cotton male sterile lines and three-line hybrid cotton.
From 2003 to 2005, in the special year of 863, he presided over the research of cotton molecular marker-assisted breeding.
From 2003 to 2005, the National Natural Science Foundation presided over the inheritance and application of late-maturing mutant traits of cotton anthers.
From 2005 to 2007, the National Natural Science Foundation supported the genetic mechanism of glyphosate-resistant mutants in cotton and their application in hybrid cotton seed production.
2005-2009, National 973 Project Year, Research on Functional Genes and Molecular Improvement of Cotton Fiber Quality Traits, Project Leader.
2002-2005, Zhejiang Key Project Year, Breeding of New Cotton Three-line Hybrid Combinations, Moderator.
From 2003 to 2005, the key project of Zhejiang Province, molecular marker breeding research of cotton resistance to verticillium wilt, was the host.
From 2004 to 2006, the key project year of Zhejiang Province, breeding and application of new non-transgenic glyphosate-resistant cotton varieties, moderator
In 2003-2005, the National Agricultural Achievement Transformation Fund, Zhejiang 905, a new cotton variety with high quality and multi-resistance, presided over the demonstration.
From 2003 to 2005, Zhejiang 905, a new cotton variety with high quality and multi-resistance, was expanded and promoted in the year of national major achievement promotion project.
From 2004 to 2006, the national year of transforming agricultural achievements into genes, the experimental demonstration of glyphosate-resistant hybrid cotton seed production technology, the host (1 item) won the prize.
Project Name: Study on New Cotton Varieties Synthesizing [AG] Complex Genome from Asian Cotton (A) and Bick Cotton (G)
Award-winning level: third prize of national invention (fourth inventor)
Award winning time:199565438+February.
Project Description: In addition to producing a large number of high-quality natural fibers, cottonseed is also rich in protein and fat, of which protein content is as high as 43-45%, which is a valuable edible protein resource. However, because cottonseed contains pigment glands and gossypol and its derivatives which are toxic to humans and monogastric animals, its high-quality protein resources are inedible. Although the whole plant has no pigment glands, its grain gossypol content is very low, which is safe to eat, but at the same time, it loses its antagonistic effect on many pests and diseases because of the low phenol content, which limits the cultivation of low phenol cotton. Gossypium bickii Prokh (G genome, 2n=26), a diploid wild cotton species originated in Australia, has a special feature of delayed formation of pigment glands in cotyledons, that is, the seeds have no pigment glands, but after germination, pigment glands containing gossypol appear in cotyledons, hypocotyls and other organs and tissues of plants. Therefore, if this trait can be transferred to cultivated upland cotton (G. hirsutum L.) and upland cotton varieties with the characteristics of seed pigment glands and plant pigment glands can be cultivated, the advantages of low-phenol cotton and phenol cotton can be combined to produce excellent pigment glands and seeds with extremely low gossypol content for comprehensive utilization without reducing the resistance to cotton diseases and insect pests, thus improving the economic benefits of cotton planting.
This achievement is a national natural science foundation project-the research topic of delayed formation characteristics of leaf glands of wild cotton seeds, with the number of 39070564. Plant chromosome engineering technology is mainly used to cross Asian cotton with homologous diploid A genome and wild Bick cotton with G genome into heterodiploid. After chromosome doubling and rooting induction, a fertile [AG] allotetraploid, Abibi Cotton, was bred, which added a new [AG] complex genome to Gossypium and enriched cotton genetic resources. This resource retains the characteristics of Beek's cotton glandless plant, and its cottonseed kernel can be eaten directly without gossypol (without detoxification). The cotton plant contains high gossypol and has strong resistance to diseases, insects, rats and other hazards. It is of great significance for the comprehensive utilization of cottonseed protein and improving the stress resistance of cotton plants, and is the first research achievement in the world. The synthesized new [AG] composite genomic materials can reproduce themselves under natural conditions, with consistent characters and stable species. Karyotype and genome analysis show that this material has species independence, which can provide scientific basis for the study of cotton seed origin and evolution. This material and its chromosome engineering technology have been widely used in cotton breeding.
Project Name: Breeding Techniques of Four New Cotton Varieties for Special Purpose
Award-winning level: the third prize of scientific and technological progress in Henan Province (third finisher)
Award winning time:199665438+February.
Project Description: Four kinds of special cotton include insect-resistant cotton, low-phenol cotton, drought-resistant alkali cotton and long-staple cotton. Breeding and popularizing these four new cotton varieties have important economic, social and ecological benefits for improving cotton planting economic benefits, reducing cotton planting costs and increasing cotton output value. However, these special cotton germplasm resources generally have the correlation between special traits and unfavorable agronomic and economic traits, and it is difficult for traditional breeding methods to fundamentally improve the production performance of these special cotton varieties. Therefore, the promotion of special cotton is limited to a certain extent.
This achievement is the main content of the national "Eighth Five-Year Plan" key project-special cotton new variety breeding. Through five years' collaborative research, an effective special cotton breeding technology system has been formed from the aspects of improving the adaptability, stress resistance and fiber quality of special cotton, including (1) breaking the bad correlation between special cotton traits and unfavorable agronomic and economic traits by means of hybridization among special cotton varieties (lines), compound hybridization and improved backcross; (2) Breeding techniques such as off-site selection identification and disproportionation selection were adopted. Improve the production adaptability and stability of existing special cotton varieties; (3) Adopting the breeding method of continuous directional screening to gradually improve the agronomic characters and stress resistance of special cotton; (4) Accelerate the breeding process of special cotton and shorten the gap between special cotton breeding and conventional cotton breeding by means of trial propagation, expanding propagation coefficient and Hainan substitution.
Through the implementation of these breeding techniques, the research team has bred and passed 28 new varieties of 4 kinds of special cotton in 5 years. The yield level of newly bred special cotton varieties reached or exceeded the existing conventional cotton production level, with excellent fiber quality, insect resistance, low phenol, drought resistance and alkali resistance and fiber quality reaching or exceeding the research requirements. Due to the remarkable improvement of the production performance of the new varieties, four new special cotton varieties have been popularized in a large area. In the past five years, the promotion area of various special cotton varieties was 10.34 million mu, creating social and economic benefits of 2 10/0.00 million yuan. In addition, the large-scale popularization of new special cotton varieties shows great social and ecological benefits: the popularization of insect-resistant cotton reduces the harm of main pests in cotton, saves the investment in pest control and improves the ecological environment in cotton areas; Low phenol cotton promotes the comprehensive utilization of cotton by-products and increases the economic benefits of cotton planting; The popularization of drought-tolerant and alkali-tolerant cotton has directly alleviated the contradiction of water shortage in cotton areas.
Project name: glyphosate-resistant mutant of cotton and its application in hybrid cotton seed production
Appraisal grade: Achievement appraisal of Zhejiang Science and Technology Department (first completed person)
Evaluation time: April 2003
Project outline: Through plant tissue culture and γ -ray mutagenesis, combined with continuous resistance screening of directional somatic cells and regenerated plants, the glyphosate-resistant upland cotton germplasm line R 1098 was cultivated. Genetic testing showed that the resistance of the material to glyphosate was controlled by a single dominant gene, and it had no cytoplasmic effect and was stable. R 1098 belongs to the middle-maturing upland cotton type, the lint yield is equivalent to that of the control variety, the fiber quality belongs to the high-quality fiber type, and it is resistant to Fusarium wilt and verticillium wilt, so it is a new excellent cotton germplasm line. The glyphosate resistance of this germplasm line has important utilization value in the utilization of cotton heterosis. The hybrid cotton seeds produced by using R 1098 as the male parent or transferring its resistance to the male parent of high heterosis hybrid combination can grow and develop normally after being treated with herbicides, while all the false hybrids die because they are not resistant to herbicides. Therefore, the glyphosate resistance of R 1098 can be used to quickly identify the purity of hybrid cotton seeds and fields, which is of great significance to the production and utilization of hybrid cotton. R 1098 is a non-transgenic glyphosate-resistant herbicide germplasm, and there is no environmental release safety problem. The breeding of this material and its application in cotton heterosis are the first time in the field of cotton breeding research at home and abroad.
The technical route adopted in this study is novel, the research method is advanced, the experimental design is reasonable, the analysis, identification and test data are complete and reliable, and the innovation is outstanding. The overall research level has reached the advanced level of similar research in the world.
(2) Cultivation of new varieties
Variety name: A new cotton variety with high protein and low phenol-China Cotton Research Institute 13.
Approved provinces and cities: Hebei province, Shandong province, and the state.
Examination and approval time: Hebei province 1990, Shandong province 1990, domestic 199 1 year.
Main contribution: the second finisher.
Variety introduction: China Cotton Research Institute 13 is a new low-phenol cotton variety with Zhong 15 1 as the material, which was bred by systematic breeding and disease resistance screening. From 1988 to 1989, the average lint yield per mu before frost was 64.3 kg, which was 95.7% of the control (Ji 8), ranking first among low-phenol cotton varieties. In the production test, the average yield per mu of lint cotton before frost was 76.4 kg, which was 4. 1% higher than that of the control, ranking first. The fiber quality was determined by Beijing Fiber Inspection Institute at 1985~ 1986. The average fiber length is 30.6 mm, the single fiber strength is 3.97 g, the fineness is 6326 m/g, the breaking length is 25. 1 km, and the maturity coefficient is 1.63. The gossypol content in cottonseed is 0.0 19%, which is lower than the standard set by the international health organization. According to the identification results of the artificial inoculation pool in plant protection room 1986 ~ 1987 of our institute, it belongs to the type of both Fusarium wilt and Verticillium wilt, and its resistance is better than that of Zhong Mian 12. 1990 passed the examination and approval of crop variety examination and approval committees in Hebei and Shandong provinces and was recognized by the state. 1986~ 1989 accumulated demonstration and promotion area 1 15000 mu.
Variety name: A new low-phenol cotton variety-Zhongmiansuo 22
Approved province and city: Hebei Province.
Approval time: 1994
Main contribution: the first finisher
Variety Description: Zhongmiansuo 22 is a new low-phenol cotton variety with high yield, stable yield, good quality, disease resistance and early maturity, which is bred with 7263-4278×[(758× Zheng 3 1) (250× Lan 5)]. 1994 was approved by Hebei Crop Variety Approval Committee in March. During the whole growth period 120~ 130 days, the boll weight was about 5.0g, the lint percentage was about 40%, and the sub-index10.5g. The yield and stability of Zhongmiansuo 22 were good, and the yields of its progeny and lint before frost were generally higher than those of the phenolic control variety 12, which overcame the previous problems. What's more worth mentioning is that the actual yield level of China Cotton Institute 22 in the harvest year is high, with great potential for high yield; However, in the poor harvest year, its yield loss is less than that of ordinary phenol cotton and low phenol cotton varieties, showing good variety adaptability. According to the test results of cotton quality supervision, inspection and testing center of the Ministry of Agriculture from 1986 to 1993 for 8 consecutive years, the span length of 2.5% fiber is 29.6mm, the uniformity is 54.2%, the specific strength is 2 1.2g/tex, and the micronaire value is 4.6. The seed quality is appraised by the Seed Inspection Room of Cotton Research Institute of Chinese Academy of Agricultural Sciences. The gossypol content in seed kernel is 0.005 1%, which is far lower than the standard set by the international health organization (0.04%). Protein content is 45.34%; The fat content is 32.2%. The incidence of Fusarium wilt was 65438 05.7%, and the disease index was 5.9. The verticillium wilt rate was 53.4%, and the disease index was 20. 1, which belonged to resistance to blight and yellowing.
Variety name: Zhejiang 905, a new cotton variety with high quality and multi-resistance.
Approved province and city: Henan Province.
Approval time: 200 1 year
Main contribution: the first finisher
Variety introduction: A new cotton variety with high quality and multi-resistance is a new cotton variety with high yield, high quality and high resistance to cotton bollworm by introducing Bt gene of Bacillus thuringiensis and CPTI gene (bivalent gene) of cowpea trypsin inhibitor into the breeding offspring produced by compound hybridization, and improving insect resistance, fiber quality and agronomic traits through conventional breeding methods. 1999~200 1 participated in cotton regional test and production test in Henan province, and was approved by Henan crop variety Committee in March 2002. In the 1999-2000 regional test of cotton varieties in Henan Province, under the condition of normal pest control, the average cotton yield before frost was 2 15.4 kg/mu, which was11.8 higher than that of the control (China Cotton Institute 19). The average lint before frost was 82.0 kg/mu, which was 6.5% higher than the control, and the yield was significantly improved. The fiber quality is appraised by the Cotton Quality Testing Center of the Ministry of Agriculture. 2.5% fiber span length is 30. 16mm, fiber uniformity is 45.54%, fiber specific strength is 22.4 cN/tex, and micronaire value is 4.3, which is a high-quality fiber type. The disease resistance was identified by the Plant Protection Room of Institute of Cotton, Chinese Academy of Agricultural Sciences. The average incidence of Fusarium wilt was 65438 09.6%, and the disease index was 8.965438 0%. The verticillium wilt rate was 42.6%, and the disease index was 28.5 1%. It belongs to the type of resistance to Fusarium wilt and verticillium wilt, and has the characteristics of resistance to seedling diseases and boll diseases.
Variety name: Zhejiang University No.3, a new cotton variety with high quality, high yield and salt tolerance.
Approved province and city: Shanxi Province.
Approval time: 2005
Main contribution: the first finisher
Variety Description: Zhejiang University No.3 (formerly known as Z9087) is a high-yield, stable-yield, high-quality and salt-tolerant hybrid combination {China Cotton Institute 10× [(Zhong 8× Zheng 3 1 )× (250× Lan 5)] F7} F6. From 2000 to 2006, 5438+0 participated in the variety comparison test, multi-point test in Henan, Anhui and Zhejiang provinces in 2002, cotton regional test in Shanxi province in 2003, and cotton regional test and production test in Shanxi province in 2004.
Zhejiang University No.3 seed cotton and lint cotton have high yield and good high and stable yield. In the strain comparison test of 5438+0 from 2000 to 2006, the average yield of lint before frost was 225.8kg/ mu, which was 15.8% higher than that of the control (Simian No.3), and the average yield of lint before frost was 96.7kg/ mu, which was 15.7% higher than that of the control, reaching a very high level. In 2002, the average lint yield before frost was 2 14.3 kg/mu in multi-point experiments (5 points) in three provinces, which was higher than that of the control (China Cotton Institute 19) 14.7%, and the average lint yield before frost was 85.4 kg/mu, which was higher than that of the control. In the regional cotton test in Shanxi Province in 2003, the average yield of cotton before frost was 245. 1 kg/mu, which was 13. 1% higher than that of the control. The average yield of lint before frost was 98.6 kg/mu, which was 32.7% higher than that of the control. In the regional cotton test in Shanxi Province in 2004, the average lint yield before frost was 2 13. 1kg/ mu, which was 98.48% of that of the control, and the average lint yield before frost was 84.4kg/ mu, which was higher than that of the control. The average cotton yield before frost in two years was 229. 1 kg/mu, which was 5.77% higher than that of the control (jinmian 3 1), and lint before frost was 9 1.5 kg/mu, which was 22. 18% higher than that of the control. In the cotton production experiment in Shanxi Province in 2004, the average yield of lint before frost was 204.6 kg/mu, 9.06% higher than that of the control, and the average yield of lint before frost was 82.6 kg/mu, 32.6% higher than that of the control, which had certain production potential. Zhejiang University No.3 fiber has excellent quality. According to the results of cotton variety testing center of the Ministry of Agriculture in 2003-2004, 2.5% fiber has an average span length of 30.4mm, fiber uniformity of 84.4%, fiber specific strength of 3 1.7 CN/tex, elongation of 6.7% and micronaire value of 4.7, which is a high-quality fiber variety. Disease resistance According to the identification results of Plant Protection Office of Cotton Research Institute of Chinese Academy of Agricultural Sciences, the average incidence of Fusarium wilt in Zhejiang University No.3 was 20.5%, and the disease index was 8.9. The incidence of verticillium wilt was 38.0%, and the disease index was 18.9. It was a variety resistant to Fusarium wilt and verticillium wilt. The results of Shanxi regional test in 2003 showed that Zhejiang University No.3 had the same disease resistance as the control (Jinmian 3 1). In addition, the experiments in Xiaoshan and Cixi, Zhejiang Province, and the indoor identification by the Institute of Crop Science of Zhejiang University showed that Zhejiang University No.3 had good salt tolerance, could grow normally at a salt concentration of 0.3~0.4%, and showed good drought resistance, so it was a salt-tolerant and drought-tolerant cotton variety.
(3) Research papers
1, Zhu, Wang, Chen Hua, Lu Changhua, Study on Late-sowing Production Performance of Low Phenol Cotton, China Cotton, 199 1, (1);
2. Zhu, Wang, Chen Hua, Lv Changhua, Preliminary Report on Breeding of Insect-resistant Low Phenol Cotton, China Cotton, 199 1, (6): 2 1 ~ 24.
3. Zhu, Zhang Bojing, Breeding and Research of New Glandular Allotetraploid Cotton Germplasm, Cotton Science, 199 1, 3 (1): 27 ~ 32.
4. Zhu,, Lv Changhua, Wang, Changes of late sowing production performance of low phenol cotton, China cotton, 199 1, 18 (1);
5. Zhu, Wang, Chen Hua, Preliminary Report on Breeding of Insect-resistant Low Phenol Cotton, China Cotton, 199 1, 18 (6): 2 1.
6. Zhu, Zhang Bojing, Study on Transformation of Delayed Occurrence Traits in Cotton Leaf Glands, Proceedings of the International Cotton Symposium, Beijing1September 1994, China Agricultural Science and Technology Press, 1993, p14150.
7. Zhu, African Cotton Insect-resistant Variety Resources, Cotton Abstracts, 1993, 1993, (5):
8. Zhu, Song, Wu Hanbei, Zhang Bojing, Study on the characteristics of delayed occurrence of leaf glands in allotetraploid Asian cotton× Bick, Cotton Science,1993,5 (1): 31~ 36.
9. Zhu Shijun, Li Baolin, Study on Gene Introduction of Glandless Plant Traits in Upland Cotton. Coton Fiber Company. , 1993,58(3): 195~200
10, Zhu, Wang, Wu hanbei, summary of early-maturing low-phenol cotton variety resources in China, crop variety resources, 1994, (3): 18 ~ 19.
1 1, Zhu Shuijin, cotton semi-mating propagation and haploid breeding, Jiangxi cotton, 1994, (3): 15 ~ 18.
12, Zhu, Wang,, Overview of new early-maturing cotton variety resources in China, crop variety resources, 1994, (3): 18 ~ 19.
13, Zhu, Wang,, A new low-phenol cotton variety-Zhongmiansuo 22, China cotton,1994,21(12): 23.
14, Zhu Shuijin, an example of plant hybrid evolution-Bike cotton, China cotton,1994,21(4): 31.
15, Zhu, Wu hanbei, Zhang bojing, study on genetic characters of three-hybrid glands between phenol and low phenol upland cotton, Cotton Science,1994,6 (increase): 19 ~ 24.
16, Zhu, Wang, Wu hanbei, effect and cause analysis of low phenol control of cotton bollworm, cotton science,1994,6 (increase): 84 ~ 85.
17, Zhu, Wang, Wu Hanbei, Study on the Occurrence and Control Effect of Cotton Bollworm with Low Phenol, China Cotton,1995,22 (1):16 ~17.
18, Zhu, Wang, Ji Daofan, 1995, Classification of Gossypium and its genome research progress, Cotton Science,1995,7 (3):1~ 6.
19, Zhu, Wang, Zhang Bojing, Synthesis and Characters of Interspecific Hybrids of Asian Cotton, Bike Cotton and Sea Island Cotton, Cotton Science,1995,7 (3):160 ~163.
20. Zhu, Wang, Wu Hanbei, Cultivation of near-isogenic lines of dominant glands in upland cotton, China cotton,1995,23 (9):14 ~15.
2 1, Zhu, Wang, Wu Hanbei, Dominant gland character effect of main economic characters of upland cotton, Jiangxi cotton, 1995, (2): 15 ~ 17.
22. Zhu, Wang, Wu Hanbei, Brief Introduction of French Low Phenol Cotton Variety Resources, China Cotton,1995,22 (2): 24.
23. Zhu, Wang, Wu Hanbei, Study on the Feeding Habit and Control Effect of Helicoverpa armigera on Low Phenol Cotton, Proceedings of Xinjiang International Cotton Symposium, China Agricultural Press, 1995, 133.
24. Zhu, Wang, Ji Daofan, Plant protease inhibitors and their application in insect-resistant genetic engineering, biotechnology,1995,5 (3):1~ 5.
25. Zhu, Current Situation, Problems and Countermeasures of Cotton Production in China, International Symposium on Resistance of Helicoverpa armigera, Beijing, 1996, 10/8 ~/0/2; [40e]: English
26. Zhu, Current Situation, Problems and Countermeasures of Cotton Production in China, International Symposium on Cotton Bollworm Resistance, Beijing, 1996,10,8 ~12; [40e]: English
27. Zhu, Ji Daofan, Advances in genetic engineering of cotton insect resistance, Advances in bioengineering, 1997, 16 (1): 36 ~ 39.
28. Zhu, Karyotype Studies of Five Australian Wild Cotton Species, Cotton Science,1997,9 (5): 248 ~ 253.
29. Zhu, Studies on Pigment Glands and Gossypol Characters of Five Australian Wild Cotton Species, Cotton Science,1997,9 (2): 84 ~ 89.
30. Zhu, Study on Plant Morphological Characteristics and Peroxidase Isozymes of Five Australian Wild Cotton Species, Acta Botanica Northwest, 1997, 17 (4): 433 ~ 438.
3 1, Zhu Shuijin, analysis of seed nutrient composition of five Australian wild cotton varieties, crop variety resources,1997,3:
32. Zhu, Histological observation of delayed formation of pigment glands in wild cottonseed leaves in Australia (Changsha Fair), Cotton Science, 1998, 10 (2): 8 1 ~ 87.
33. Zhu, Effect of Screening Varieties Resistant to Verticillium Wilt under All Adversity, China Cotton,1998,25 (5)
34. Zhu, the dynamics of gossypol in wild cottonseed leaves in Australia and its relationship with the morphology of pigment glands, Cotton Science, 1999,11(4):169 ~173.
35. Genetic study on interspecific hybrids of Zhu Shuijin, Ji Daofan, upland cotton and Stiti cotton and their pigment gland characters, Acta Genetica,1999,26 (4): 403 ~ 409.
36. Jon Chu, Ji Dongfeng, Interspecific Hybridization between Willis Cotton and Upland Cotton and Inheritance of Pigment Gland Traits, China Journal of Genetics,1999,26 (3): 257 ~ 264.
37. Genetic Effects of Different Genes of Pigment Glands in Zhu, Ji Daofan and Upland Cotton on Delayed Formation of Pigment Glands in Cotton Leaves, Acta Crops,1999,25 (5): 585 ~ 590.
38. Zhu, Cultivation and Research on Verticillium Wilt Resistance of Near-isogenic Lines in Upland Cotton (Revised Draft), China Cotton,1999,26 (3);
39. Induction of drug resistance of Helicoverpa armigera by Zhu Shuijin, cotton pigment glands and gossypol (English), unfinished, 26 (3): 23 ~ 24.
40. Zhu, Ji Daofan, Establishment of cotton root tip culture system in vitro, Cotton Science, 2000, 12 (6): 288 ~ 293.
4 1, Zhu, Ji Daofan, Liu Shengan, Wang, Induction of resistance of cotton pigment glands and gossypol to Helicoverpa armigera and analysis of esterase isozyme, Cotton Science, 2000,12 (1):12 ~16.
42. Effects of vermilion, cotton pigment glands and gossypol on the growth and drug resistance of Helicoverpa armigera, China Agricultural Science, 200 1, 34 (2): 157 ~ 162.
43. Zhu, Ji Daofan, Dynamics of Gossypol in Cotton Seedlings and Roots in Vitro, Cotton Science, 200 1,13 (4):195 ~199.
44. Zhu, Ji Daofan. Research progress of Verticillium dahliae and its resistance genetic breeding. Cotton Science, 200 1,13 (2):116 ~120.
45. Zhu, Ji Daofan, Genetic analysis of delayed formation of pigment glands in wild cottonseed leaves in Australia, Science Bulletin, 200 1, 46 (2): 132 ~ 136.
46. Jon Chu, Ji Dongfeng, Inheritance of delayed glandular development in Australian wild cotton, China Science Bulletin, 200 1, 46 (14):1168 ~1/73.
47. Zhu, Ji Daofan. Effects of pigment glands and gossypol on shoot tip culture of upland cotton. Acta Crops, 200 1, 27 (6): 737 ~ 742.
48. Zhu, Ji Daofan, Liu Shengan, Wang, Effects of Pigment Glands and Gossypol on Callus Induction and Growth of Upland Cotton, Science Bulletin, 200 1, 46 (16):1380 ~1383.
49. Zhu Shijun, Ji Dongfeng, Effects of pigment glands and gossypol on somatic cell culture of upland cotton, Science Bulletin, 200 1, 46(23): 1975~ 1979.
50. Zhu, Ji Daofan, Breeding of Late-maturing Mutants in Upland Cotton and Preliminary Report on Their Biological Characteristics, Cotton Science, 200 1, 13 (3): 193.
5 1, Zhu shijun, fan wenping, Ji Dongfeng, study on molecular marker-assisted selection of verticillium wilt resistance in upland cotton, journal of the second national plant genome university, Hangzhou, 200 1.
52. Ren, Hu Jiashu, Zhu, Cotton Verticillium wilt resistance and peroxidase isozyme analysis, Cotton Science, 2002, 14 (5): 273 ~ 276.
53. Study on pollen abortion mechanism of Hu, Zhu, F 1 diploid sterile line [1], Cotton Science, 2002, 14 (6): 330 ~ 335.
54, Zhu Shijun, Fan Wenping, Ji Dongfeng, Study on molecular marker-assisted selection of verticillium wilt resistance in upland cotton. Cotton Science, 2002, 14 (increase)
55. Zhu, Tong, Ji Daofan, Induced Effect of Monocrotophos on Verticillium Wilt of Upland Cotton, Cotton Science, June 2003.
56. A new cotton variety with high quality and multi-resistance, Zhejiang 905, China Cotton, 2003,30 (2): 31~ 32.
57. Histological observation on morphogenesis of pigment glands, Zhu Shuijin, Hu, Ji Daofan, Bikemian and viscose cotton, Cotton Science, 2003,15 (1):17 ~ 22.
58. Zhu, Hu, Ji Daofan. Morphogenesis of pigment glands in cotton plants and dynamics of gossypol. Acta Crops, 2004,30 (2):100 ~104.
59. Genetic study on verticillium wilt resistance of upland cotton and island cotton, Cotton Science, 2003, 15 (1): 3 ~ 7.
60, Lu Dezhao, Zhu, Wang, Yan Meixian, Comparison of methods of BADH transgenic rice, China Rice Science, 2003, 17 (4): 323 ~ 327.
6 1, Luan Qifu, Zhu, upland cotton recombinant inbred line HM 188 and its characters, Cotton Science, 2003, 15(4):
62. Zhu, Jing Er Wang, Screening of glyphosate-resistant mutants in cotton and their application in heterosis utilization, Cotton Science, 2003, 15 (4): 227 ~ 230.
63. Gao Zhu Ji Daofan, Cytogenetic Study on Four-element Hybrid F 1 synthesized by Four Cultivated Cotton, Cotton Science, 2003, 15 (5): 259 ~ 263.
64. Wang, Zhu,,, Effects of space flight on biological characteristics and SOD activity of eggplant SP 1, Journal of Nuclear Agronomy, 2004, 18(4)303~306.
65. Zhu, Gao, Ji Daofan, Breeding of Cotton Germplasm Line 5629 with Resistance to Verticillium Wilt and Low Phenol and Study on Resistance Mechanism, Cotton Science, 2004, 16 (5): 307 ~ 3 12.
66. Zhu Leidi, Jiang Yurong, Ji Daofan, Breeding and Genetic Study of Pigment Glands in Cotyledons of Upland Cotton, Science Bulletin, 2004,49 (19):1987 ~1992.
67. Zhu Shijun, Lei Dinan, Jiang Erjie, Ji Dongfeng, Breeding, Introgression and Inheritance of Delayed Morphological Development of Cotton Glands in Cotton Germplasm Resources, Science Bulletin, 2004,49 (23): 2470 ~ 2467.
68. Reddy N, Zhu Shijun, Jiang YR, Tong. Genetic transformation of cotton mediated by pollen tube pathway. Go on. Proceedings of the International Symposium on "Sustainable Cotton Production Strategy-Global Perspective" I. Crop Improvement. 23-25 September 2004, Dawood, India.
69. Zhu Shijun, Jiang Yongnian, Lei Dinan, Ji Dongfeng, Study on the method of gene introduction of delayed pigment gland development in upland cotton, plant breeding, (2005, in press)
70. Jiang Yurong, Zhu, Ji Daofan, Relationship between Plant Tissue Structure, Biochemical Metabolism and Verticillium Wilt Resistance in Upland Cotton, 2005, Acta Crops, 2005,365,438+0 (3)
7 1, Gao, Zhu, Ji daofan, cytogenetics and genetic relationship of four cultivated cotton interspecific hybrids F 1, Acta Genetica, 2005,32 (7 ~ 8).
72. Reddy N, Patil VC, Zhu Shijie, Planting to Catch More Sunlight, Reza, 2005,21(1): 7.
73. Control Effect of Crude Extracts of Phytolacca acinosa on Tobacco Mosaic Virus, Southwest Agricultural Science and Technology Service, 2005,18 (2):168 ~171.
(4) publishing works
1. experimental teaching of genetics (2 1 century textbook). China Agricultural Publishing House, 2005 (Editor-in-Chief)
2. Breeding of short-season cotton. China Agricultural Publishing House, 2005 (co-author)
3. Plant biotechnology. Published by Science Press, 2004 (co-author)
4. Cotton genetic breeding. China Agricultural Publishing House, 2002 (co-author)
5. Crop Breeding (2 1 century textbook). China Agricultural Publishing House, 2002 (co-author)
6. Encyclopedia of agriculture. Crop rolls. Cotton. China Agricultural Publishing House, 2002 (co-author)
7. Crop seed production and quality control technology. Zhejiang University Press, 200 1 (co-author)
8. Contemporary world cotton industry. China Agricultural Publishing House, 1995 (co-author)
9. Breeding of glandless cotton and comprehensive utilization of cottonseed. Agricultural Press, 1988 (co-author)