From 65438 to 0998, Liu Jiaoshou's research group continuously participated in the annual meeting of ASLMS (the highest international conference on laser medicine). From 65438 to 0999, the report of the research group became one of the eight hot topics in this group meeting. In 2000, the report of the research group on the membrane mechanism of cells stimulated by low-intensity laser was nominated as the best basic research paper in the whole conference and the excellent paper in the group meeting. 200 1 the report of the research group was selected into the Mini-Talk conference, ranking first. Graduate students of the research group have been supported by the Summer Research Fund for three consecutive times. Lasers Surgery Medicine is the journal of laser medicine with the highest impact factor (3.275 in 20 1 1) included in SCI. 1. 1998-2000, the national natural science foundation project: the study on the signal transduction mechanism of low-intensity He-Ne laser by human neutrophils has been completed.
2. 1998-2000, funded by Fok Ying-tung, Ministry of Education: Study on signal mechanism of low-intensity He-Ne laser transduced by human neutrophils.
3. From 2000 to 2002, the Summer Research Fund of the Laser Medical Association of the United States funded three graduate programs: the study on the mechanism of low-intensity He-Ne laser activating neutrophil NADPH oxidase complex, the influence of anesthetics on the normal skin fibroblasts of pigs irradiated by laser, and the biological regulation of light on fibroblasts in the long-term effect of laser surgery, which has been completed.
4.2001-In 2003, he co-chaired the Guangdong Natural Science Foundation project: Optical transmission in different fields, mainly responsible for the basic research of photobiological regulation, which has been completed.
5. In 2002-2004, the National Natural Science Foundation of China completed the research on activation of human neutrophil tyrosine protein kinase by low-intensity He-Ne laser.
6. In 2002-2004, he completed the "Thousand Hundred and Ten Projects" excellent talents training fund (provincial level): "Study on the mechanism of delaying the aging of human diploid fibroblasts by low-intensity He-Ne laser".
7. From 2005 to 2007, the National Natural Science Foundation of China completed the study on the mechanism of LED red light inhibiting the apoptosis of PC 12 cells induced by amyloid 25-35.
8.20/20091kloc-0/National Natural Science Foundation Project "Study on Mechanism of Low Intensity 650 nm GaInP/AlGaInP Semiconductor Laser Promoting the Formation of Neutrophil Extracellular Sterilization Network" has been completed. 1. Interaction between radiation and matter: The quantum characteristics of time are revealed for the first time, and the corresponding quantum theory of time (RMT) is established.
2. Non-vibration transition theory (NRT) of biological effects of low intensity laser is established by RMT.
3. Residence Biology: RMT and NRT put forward the physical amplification model and time parallelism of physical signals, established the time method (BIT) for extracting biological residence, and revealed the relationship between the most probable life expectancy Y and the best age H: Y=2H+ 1.
4. Basic theory of TCM: combine process time with Yin and Yang, develop TCM methods, and establish the theory of Yin and Yang's virtual and real time; The biological coherence of acupoints is put forward, the time sub-model of sensation transmission along meridians is put forward, and the time theory of meridian phenomenon is established.
5. Transmission optics: Maxwell equation is written as Schrodinger equation, and quantum mechanics theory of beam propagation is established by quantum mechanics method. Among them, the time quantum theory of photobiology was jointly evaluated as the world's outstanding academic achievement by the International Center for Economic Evaluation (Hong Kong), the Hong Kong Weibao Society and the World Chinese Academic Achievement Jury, and the research on the time theory of Yin and Yang of TCM won the gold medal of Zhang Zhongjing's innovation and invention in the second global Chinese medical conference in 1999. Time quantum theory of laser chemistry won the second prize in the second Huazhong Science and Technology Promotion Conference. Nearly 60 papers have been published in Laser Sur Med, Phys Rev E, China Science and other magazines; SCI has published more than 40 papers and been cited for more than 20 times.
6. The phenomenon of cooperative light signal transduction in mammalian non-visual cells was found. Photobiomodulation (PBM) is a non-destructive regulation of biological system by laser or monochromatic light (Li). Li Can is divided into low-intensity irradiation (10w/cm) and medium-intensity irradiation (0. 10 ~ 1.0 w/cm), among which low-intensity and short-time MIL irradiation is called low-level irradiation (LLL). Led by Academician Liu Songhao, Professor Liu Chengyi's Laser Sports Medicine Laboratory (LSM) has been engaged in the research of 16 years since it put forward PBM internationally, and has been insisting on studying the biological effects of LIL. The laboratory first proposed the hormone-like model of LIL, and gradually developed it into a bioinformatics model. 200 1 was directly confirmed by the experiment of cell light signal transduction, and won the best excellent photobiostimulation paper award at the annual meeting of the American Laser Medical Association. Recently, functional networks that place signal pathways in cells have further discovered redundancy mechanisms. Over the years, it has been found that LIL can affect many cell physiological functions through invisible light regulation, inhibit cell apoptosis, resist oxidative stress, promote cell proliferation, and induce healthy neutrophils to form extracellular bactericidal nets. In the aspect of dose relationship, LSM first found the dose band effect, and found that the initial process of PBM was the key process of PBM, and further gave the quantitative dose relationship. Combined with the study of dose relationship of FSH, the adaptive characteristics of LIL were found. These findings not only deepen the research of PBM, but also provide a solid theoretical basis for the clinical application of LIL. LSM suggested that LIL could be used as an adjuvant therapy for diseases such as Alzheimer's disease, sports injury and viral influenza.
7. The book "Low-intensity laser intranasal irradiation therapy" was published in Chinese and English in People's Military Medical Publishing House on 20 10. This book is bilingual and professional. This paper systematically introduces the low-intensity laser intranasal irradiation therapy and its home use method. Liu Jiaoshou used the products of Tianjin Huaxin Medical Company for basic research.
8. The concepts of functional homeostasis and success pressure are put forward. Early research on PBM focused on whether PBM was observed or not. Professor Liu Chengyi combined negative feedback with function and stress, put forward the concepts of function-specific steady state (FSH) and successful stress, and divided PBM into low-level PBM and high-level PBM, which fundamentally solved the problem of when PBM can be observed.
However, in recent years, it has been found that it is not enough to talk about the existence of PBM in a certain function. Only by putting functions into the functional network of biological systems such as cells or tissues can we understand PBM more comprehensively. With the introduction of functional networks, PBM can be further divided into direct PBM (dPBM) and indirect PBM (iPBM).
9. Light signal transduction phenomenon of neutrophils
There are a large number of receptors for signal molecules such as peptide hormones on the cell membrane. Signal transduction mediated by membrane receptors includes the recognition of signal molecules, the transformation and transduction of information and the activation of effectors, among which protein kinases that realize information transformation and transduction constitute signal transduction pathways. Signal pathways can be divided into two categories: the first category is the signal pathway mediated by Gs protein (pathway 1), and the second category is the signal pathway mediated by Gq protein, Gi protein or receptor-related enzymes (pathway 2). There are receptors on the cell membranes of visual cells and retinal ganglion cells that can vibrate with photons, and the signal transduction mediated by them is called light transduction: PTD).
The phenomenon of reactive oxygen species (ROS) produced by non-mitochondria (PMN) of neutrophils is called respiratory burst. Previous researchers (Schepetkin et al. 1994, Karu 1998) found that low-intensity He-Ne laser irradiation (LiHNL) can regulate the ROS production of PMN. Our research shows that (Duane et al 200 1), LIHNL can induce PMN respiratory burst (PMN1); In addition, LIHNL induced PMN respiratory burst through pathway 2 (PMN2). The conclusion of my paper (Duane et al. 200 1) was cited by him 10 times, among which PMN 1 was cited by Lion Fighter et al. (2003), Klebanov et al. (2005) and Zan-Barr et al. (2005) as typical examples to study the function of LIL cells, and PMN2 was cited by SCH respectively. When enumerating the representative papers discussing the LIL mechanism, the papers of Karu et al. (Derkacz et al., 2005, Bisland et al., 2006) and those of Karu and Alexandratou et al. (Zan-Bar et al., 2005) are cited side by side with other papers. When discussing the mechanism of ROS-mediated PBM, Hamblin et al. (2006) cited the work and works of Lubert et al. My paper was published at 5438+0 in August 2006, and our report at the ASLMS annual meeting in April of the same year attracted wide attention from the representatives of the biostimulation group. Dr. Hode, one of the authors of the international website on research and application of low-intensity laser therapy (Tuné r et al.1999), highly appreciated our theoretical work, and Dr. Lanzafame, former chairman of ASLMS, Mark and Dakota Prize winner, found our work exciting (Liu Chengyi et al. 200 1).
And LIHNL are non-oscillatory (Li et al. 2003). In order to distinguish it from PTD, we call PTD caused by non-specific vibration as non-specific PTD (NPTD). Shefer et al. (200 1) also found the NPTD of skeletal muscle satellite cells. Subsequently, papers on NPDT were published one after another (Shefer et al. 2003, Advanced 2006). LIHNL's research on PMN effect is based on intravascular low-intensity laser irradiation of blood: Ilib (Schepetkin et al. 1994). ILIB was first put forward by Americans and became popular in Russian and China. Although there are many basic researches in Russia (Schepetkin et al. 1994, Karu 1998), most of the high-level basic researches are in China (Tong et al. 2000, Duan et al. 200 1, Mi et al. 2004). Duane et al. 200 1 and Shefer et al. (200 1) cited the same number of times in SCI (both 6 times), and tied for the first place in the citation number of NPTD research papers.
10. Light signal inhibits apoptosis of PC 12 cells induced by amyloid 25-35.
Cells can die through necrosis, apoptosis, non-apoptotic programmed death and aging. Apoptosis of PC 12 cells induced by amyloid protein (Aβ)25-35 is one of the cell models of Alzheimer's disease. We use the wavelength of 640? Irradiated with 15nm red light emitting diode array (RLED), it was found that RLED could inhibit the apoptosis of PC 12 cells induced by Aβ25-35. The American Society of Surgeons attached great importance to this research achievement, and participated in the annual meeting of the American Society of Surgeons, which was funded by international travel expenses. Laser Surgery Medicine was published in the form of a quick report (Duan et al. 2003). This is the only paper published in Laser Surgery in the form of a research bulletin so far. Santana-Blank et al. (2005) cited this research result, which not only pointed out its significance for the treatment of AD, but also made a preliminary mechanism discussion. Although Yamamoto(2000) published the effect of low-intensity GaAlAs semiconductor laser (905nm) on neurotransmitter secretion and ion channels in PC 12 cells, our paper is the only journal paper to study the effect of LIL PC 12 cells. Although some people have studied the inhibitory effect of LIL on apoptosis, our paper is the only one to study the inhibitory effect of LIL on apoptosis in nerve cell model. So far, there is no effective prevention and treatment for AD. PBM is a very safe rehabilitation method, and our research provides a basis for direct LIL irradiation of skull to prevent and treat AD.
1 1.
The regulation of light on cell function can be divided into two categories. Specific pathways are mediated by endogenous photosensitizers that vibrate with light (Zan-Bar et al., 2005), such as cytochrome c oxidase (Karu 1998), hemoglobin (Mi et al., 2004) and endogenous porphyrin (Lion Fighter et al., 2003), while non-specific pathways are mediated by endogenous photosensitizers that cannot vibrate with light. 2004)。 The specific pathway mediated by cytochrome c oxidase was first proposed by Karu( 1998), and was completely confirmed by experiments recently. The nonspecific pathway mediated by cell membrane receptors was first proposed by us (Liu et al. 1996), and the peptide hormone model of laser biostimulation (Liu et al. 1996, et al. 1997a) developed into the biological information model of laser biostimulation (et al. 1997b, et al. 2003).
Karoo (1998) summarized the phenomenon of PBM from the perspective of redox potential (RDP). PBM has no effect on cells with normal RDP, and the lower RDP, the stronger the effect of PBM. BIMP demonstrated from the point of view of quantum mechanics. The probability of non-vibrational interaction between a single molecule and photons is very small. Nonspecific pathways depend on the state of the cell. Cells with normal function are in a steady state, and cells far from the steady state are abnormal. Only the membrane receptors of cells far away from the steady state are in superradiation state, and the non-vibrational interaction between a single receptor molecule and photons can be amplified by the square of the number of receptors (about 104) (about 108). Therefore, PBM can promote the cells far away from the steady state to return to the steady state and has the function of cell repair (Liu Chengyi et al. 2006).
The special changes in the cell membrane receptor configuration required to start the signal pathway can be realized through the coupling of intramolecular electron motion and nuclear motion. According to the non-* * vibration activation mode of light on electron motion, BIMP extends monochromatic light to long-wave ultraviolet UVA(320-400nm) and short-wave infrared IRA(700- 1000nm), and divides it into two categories: cool colors such as UVA, purple, blue and green, and warm colors such as IRA, red, orange and yellow. On the premise that monochromatic light does not cause cell damage, BIMP divides the dose of monochromatic light into several dose segments from low to high, which are respectively expressed as dose segment n (n = 1, 2, ...). Within the dose range (2n- 1), BIMP is expressed as BIMP(2n- 1), and BIMP is expressed as bimp. Cool color excitation pathway II. In the dose range of 2n, BIMP is represented as BIMP2n, warm color excitation pathway 2 and cold color excitation pathway 1.
It is not only directly supported by visible light (Duan et al. 200 1, Schaefer et al. 200 1 & 2003, NPTD experiment 2003, Advanced 2006, UVA(Bode et al. 2003) and IRA(Schieke et al. 2002), but also indirectly supported by experiments at the cell level, which is completely consistent with the theory of traditional Chinese medicine (Liu et al. Related papers directly stated by Liu et al. (1996, 1997), (1997a &; B) was cited by CNKI 1, 5, 8, 13 times respectively. In all the work related to BIMP, SCI included 26 articles, SCI cited 7 times, two comprehensive papers in SPIE's Mechanism of Low Light Therapy were cited 1 time, European monograph was cited 1 time, and CNKI was cited 5 1 time. As a direct verification of, (paragraph 2001&; 2003) was introduced in the first two sections. In addition, Gao Xiaoling et al. (1999) cited BIMP's explanation of the experimental results; Xu Chuanshan et al. (2000) summarized BIMP and emphasized the importance of studying low-intensity laser signal transduction. Yang Zaifu and others (2002) summarized BIMP, and they pointed out that the contradiction between BIMP and experiment can be explained by the monochromatic light specificity and cell specificity of PBM.
BIMP was nominated for the best basic scientific paper in ASLMS 2000, and won the best biostimulation paper award in the name of master student Duan Rui (Liu Chengyi et al. 2000). The full text was revised and included in the monograph edited by the President of the European Medical Laser Federation (Liu et al. 2003).
Effect of anesthetic on PBM
Cell and clinic have proved that PBM can promote wound healing, but animal experiments for more than 30 years have never reached a consistent conclusion. From the perspective of anesthetics, we found that the effectiveness of animal experiments may be related to the types of anesthetics used to make wounds (Li et al. 200 1), which was discussed as a hot topic in the meeting of biological stimulation group at ASLMS 200 1 annual meeting, and experimental research was funded in summer (et al. 200 1). We studied the effect of ketamine on the proliferation of fibroblasts promoted by LIHNL, and confirmed that ketamine can completely inhibit the proliferation of fibroblasts promoted by LIHNL, which provided a cellular basis for further explaining the debate for more than 30 years (Li Yan et al., 2004). ASLMS attached great importance to this result, and international travel expenses funded Li Yan to attend the meeting (Li Yan et al. 2002, Li et al. 2002).
13. Photobiological adjustment of the edge of intense laser beam
Laser surgery is the fastest developing field of laser medicine. The immediate effect of most surgical lasers is similar, but the long-term effect is quite different. We divide the high-intensity laser beam into the middle part and the low-intensity part at the edge. Assuming that there is PBM at the edge of high-intensity laser beam, the long-term effect of surgical laser is explained by BIMP, which was highly valued at ASLMS2002 annual meeting in 2002. This paper (Liu et al., 2002a) was promoted from the submitted biostimulation meeting to the first report of the small meeting summary meeting I for all delegates, and was invited to publish the full text in the online publication Laserexpress. This research project was supported by summer students of American Language and Literature Society (Li Yan et al., 2002). "Low-intensity laser effect in laser surgery" is listed as a recent priority research field of National Natural Science Foundation (Xie Shusen et al., 2004). Taking single-layer fibroblasts as a model, the damage effect of Nd:YAG laser and its frequency doubling green laser beam center and the non-damage effect of edge on undamaged cells were studied. It is found that the latter is the same as Huang 2005, which supports the hypothesis of intense laser beam edge from cell model experiments and provides a cellular basis for explaining the long-term effect of surgical laser. 14. Schrodinger formal theory of beam propagation transforms Maxwell equation into Schrodinger equation, obtains Hamiltonian and wave function of quantum mechanics, and establishes Schrodinger formal theory of beam propagation by using quantum mechanics method. The average value method of quantum mechanics is used to redefine the beam propagation parameters, and Heisenberg image is used to study the evolution of operators, and real refractive index and complex refractive index media are obtained respectively (Liu et al. 2002b &: C). It is proved that the conservation of (effective) quality factor is a necessary and sufficient condition for the existence of (effective) ABCD matrix. From the conservation of (effective) quality factor, the (effective) ABCD matrix and its diffraction integral are obtained, which are the beam propagation and diffraction integration of biological tissues. Published papers 10 were included in SCI, cited by SCI for 8 times and cited by CNKI for 2 times.