agar eosin methylene blue agar medium.
According to the query on Baidu Encyclopedia, EMB agar medium is the abbreviation of EMB (Eosin Methylene Blue) agar eosin methylene blue agar medium.
It is a medium used to observe whether acid is produced after a certain sugar is decomposed.
This medium contains a protein-like amino acid mixture, yeast extract, an appropriate amount of inorganic salts, 0.04% eosin Y, 0.0065% methylene blue and sugar for testing.
It is commonly used for the isolation and identification of Gram-negative enteric bacteria in food, dairy products, water sources and pathogen samples.
When using this medium for flat culture, if the sugar is decomposed, the colony will turn dark purple; if it is not decomposed, the colony will turn light pink.
In 1947, J. Lederberg used this medium to test the fermentability of various sugars when conducting conjugation experiments with Escherichia coli.
It was later used to conduct many genetic experiments on E. coli.