With the launch of HPV vaccine, people are paying more and more attention to HPV.
So what methods should be used to screen for HPV currently? 1. Cytological testing The Pap smear method can detect the characteristic "knockout cells" caused by HPV infection through Pap staining.
Its advantages include: high diagnostic specificity, cheapness, and ease of census; disadvantages include: low accuracy, low sensitivity and low repeatability, strong subjectivity, high false negative rate, inability to classify, and a certain rate of misdiagnosis and missed diagnosis.
2. Serological testing Serological testing mainly analyzes human serum to see whether there are HPV antibodies to achieve the purpose of detection.
The advantage is that the operation and principle are clear and simple. The disadvantage is that due to the certain hysteresis of the human body's immune response, some infected persons with incubation period and immune unresponsiveness may be missed in such detection of antigens and antibodies.
3. Genetic testing Genetic testing includes hybrid capture 2nd generation technology (HC-Ⅱ), real-time fluorescence quantitative PCR technology (Cobas 4800), enzyme digestion signal amplification method (Cervista HPV), and gene chip method.
Among them, real-time fluorescence quantitative PCR technology combines the hybridization of specific probes during DNA amplification, further improving the sensitivity and specificity of the experiment.
It can use fluorescence to monitor the DNA amplification process in real time, making the results accurate and reliable, and is very suitable for large-scale clinical screening.
Human papillomavirus (HPV) is a genus Papillomavirus A belonging to the family Papovaviridae. It is a spherical DNA virus that can cause the proliferation of squamous epithelium in human skin and mucous membranes. Currently, more than 130 different species have been isolated.
The types cause different clinical manifestations.
Different types of HPV cause different diseases. The current screening method recommends the use of genetic testing, which can clarify which type of HPV the infection is, enable corresponding intervention, and have a clearer understanding of one's physical condition.