Metagenome detection is to do the genome of environmental microorganisms. The key lies in the macro environment, including the environment in the human body and the natural environment. By sequencing all organisms in a system at the genome level, the species diversity of individuals in specific situations is analyzed. At present, the number of pathogens usually covered exceeds 10000. The whole exon detection, emphasizing comprehensiveness, is a way of high-throughput sequencing. Generally speaking, in vitro detection refers to the process of hybridizing the coding region sequence of human genome with a specific exon capture kit, and then sequencing it with high-throughput sequencing instrument. The results of exon sequencing are often used to analyze the mutation of pathogenic genes in some rare genetic diseases or polygenic genetic diseases.